Journal: International Journal of Surgery (London, England)

Article Title: Tibial cortex transverse transport surgery improves wound healing in patients with severe type 2 DFUs by activating a systemic immune response: a cross-sectional study

doi: 10.1097/JS9.0000000000001897

Figure Lengend Snippet: The design and workflow of this study. This study mainly contained four parts. First, 68 patients with unilateral or bilateral DFUs were treated with TTT surgery, and samples at six key time points were collected for the study. Second, label-free proteomic analysis of immunomodulatory proteins and regulatory pathways ( N =30, TTT 0 , TTT 3 , TTT 7 , TTT 14 , TTT 21 , and TTT 35 ) was performed. Third, skin tissue samples from three key time points were collected for spatial metabolomics to validate the immunological metabolites ( N =7, TTT 0 , TTT 14, and TTT 21 ). Next, the specific immune biomarkers were validated in the clinical laboratory (TTT 0 , TTT 3 , TTT 7 , TTT 14 , TTT 21 , and TTT 35 ). In addition, transcriptomics analysis for skin tissue samples was applied to screen out differential genes and signal pathways in regulating the immune response and wound healing ( N =3, TTT 0 , TTT 14 , and TTT 21 ). Finally, the integration of data from multiomic and clinical immune biomarker analyses was applied to investigate possible immune responses. N, the number of patients; TTT 0 , 1 day before the TTT external fixator was fixed; TTT 3 , 3 days after the TTT external fixator was fixed; TTT 7 , 7 days after the TTT external fixator was fixed; TTT 14 , the day at which the upward movement was completed; TTT 21 , the day at which the downward transfer was completed; TTT 35 , the second day after the TTT external fixator was removed. DFU, diabetic foot ulceration; TTT, tibial cortex transverse transport.

Article Snippet: The analytical platform used for proteomic analysis was from Dashuo Biotech Co. Ltd (Dalian, China), the spatially resolved metabolomics platform was from MetWare Co. Ltd (Wuhan, China), and the transcriptomics platform was from Kangxiang Biotechnology Co. Ltd (Shanghai, China).

Techniques: Biomarker Assay

Journal: International Journal of Surgery (London, England)

Article Title: Tibial cortex transverse transport surgery improves wound healing in patients with severe type 2 DFUs by activating a systemic immune response: a cross-sectional study

doi: 10.1097/JS9.0000000000001897

Figure Lengend Snippet: Spatially resolved metabolomics analysis of DFU skin tissues. (A) Crucial processing of skin tissue samples for spatial metabolomics analysis, including H&E staining of skin tissue sections, DHB matrix spraying of frozen sections, and metabolite spatial distribution with Gaussian smoothing. (B and C) OPLS-DA of spatial metabolomics datasets. (D–F) Representative mass spectra of DFU skin tissue (TTT 0 , TTT 14 , and TTT 21 ). (G–I) The bar diagram shows the expression levels of differentially abundant metabolites in glycerophospholipid, fatty acid, and glycerophospholipid metabolism pathways at TTT 14 and TTT 21 compared with TTT 0 . A total of eight differentially abundant metabolites were identified in TTT 0 , TTT 14 , and TTT 21 , and their change trends were statistically analyzed. (J–L) Bar plot of KEGG pathway enrichment analysis for the differentially abundant metabolites. The glycerophospholipid metabolism pathway was validated in DFU skin tissues. ** P value <0.005; * P value <0.05. DFU, diabetic foot ulceration; KEGG, Kyoto Encyclopedia of Genes and Genomes; TTT, tibial cortex transverse transport.

Article Snippet: The analytical platform used for proteomic analysis was from Dashuo Biotech Co. Ltd (Dalian, China), the spatially resolved metabolomics platform was from MetWare Co. Ltd (Wuhan, China), and the transcriptomics platform was from Kangxiang Biotechnology Co. Ltd (Shanghai, China).

Techniques: Staining, Expressing

Journal: International Journal of Surgery (London, England)

Article Title: Tibial cortex transverse transport surgery improves wound healing in patients with severe type 2 DFUs by activating a systemic immune response: a cross-sectional study

doi: 10.1097/JS9.0000000000001897

Figure Lengend Snippet: Spatial expression images of differentially abundant metabolites in DFU tissue sections based on spatially resolved metabolomics data (intensity in color scale is relative value). (A) Expression and spatial distributions of theobromine, levocarnitine propionate, canthaxanthin A, and triacetin at TTT 14 compared with those at TTT 0 . (B) Expression and spatial distributions of elemonic acid and camelliagenin at TTT 21 compared with those at TTT 0 . (C) Decreased concentrations of L-palmitoylcarnitine and stearoylcarnitine in DFU tissues from TTT 14 and TTT 21 patients compared with those of TTT 0 patients. * P value <0.05, ** P value <0.01. DFU, diabetic foot ulceration; TTT, tibial cortex transverse transport.

Article Snippet: The analytical platform used for proteomic analysis was from Dashuo Biotech Co. Ltd (Dalian, China), the spatially resolved metabolomics platform was from MetWare Co. Ltd (Wuhan, China), and the transcriptomics platform was from Kangxiang Biotechnology Co. Ltd (Shanghai, China).

Techniques: Expressing

Journal: Data in Brief

Article Title: Spatially resolved metabolomic dataset of distinct human kidney anatomic regions

doi: 10.1016/j.dib.2024.110431

Figure Lengend Snippet:

Article Snippet: A dataset of spatially resolved metabolomics on human kidney anatomic regions (Original data) (Mendeley Data).

Techniques: Mass Spectrometry

Journal: Frontiers in Oncology

Article Title: Spatial metabolomics in head and neck tumors: a review

doi: 10.3389/fonc.2023.1213273

Figure Lengend Snippet: Comparison of different platforms of MSI used for spatial metabolomics.

Article Snippet: They have conducted Spatial metabolomics (SM), Spatial Lipidomics (SL) and Spatial Transcriptomics (ST) analysis on frozen cancer tissue sections from patients with gastric adenocarcinoma, and depicted spatially-resolved maps of metabolites, lipids, and gene expression patterns both in tumor cells and normal cells.

Techniques: Comparison, High Throughput Screening Assay, Imaging

Journal: PeerJ

Article Title: The burgeoning spatial multi-omics in human gastrointestinal cancers

doi: 10.7717/peerj.17860

Figure Lengend Snippet: Specific molecular markers of interest to diagnose or treat specifically GI cancer revealed by the modern multi-omics techiques.

Article Snippet: Gastric cancer , Microarray-based spatial transcriptomics (ST), in addition to the mass spectrometry imaging-based spatial metabolomics (SM) and lipidomics (SL) , Postoperative cancer tissue from seven male patients diagnosed with gastric adenocarcinoma , 2023 ( ) .

Techniques: Biomarker Discovery, Immunohistochemistry, Immunofluorescence, Western Blot, Spatial Proteomics, Single-cell Transcriptomics, Diagnostic Assay, RNA Sequencing, Sequencing, In Situ Hybridization, Immunoprecipitation, Reverse Transcription